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Related Experiment Videos

Promoter usage and alternative splicing.

Alberto R Kornblihtt1

  • 1Laboratorio de Fisiologia y Biologia Molecular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, 2 piso, C1428EHA Buenos Aires, Argentina. ark@fbmc.fcen.uba.ar

Current Opinion in Cell Biology
|May 20, 2005
PubMed
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Alternative splicing (AS) is crucial, impacting 60% of human genes and proteomic complexity. Transcription significantly influences AS through promoter effects and RNA polymerase II elongation control.

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Expression Regulation

Background:

  • Alternative splicing (AS) is a key mechanism generating proteomic diversity from a limited gene set.
  • Mutations in AS regulatory sequences are implicated in numerous human diseases.
  • AS is intrinsically linked to the transcription process.

Purpose of the Study:

  • To highlight the renewed interest in alternative splicing.
  • To elucidate the mechanisms by which transcription influences alternative splicing.
  • To underscore the role of transcription-splicing coupling in gene expression.

Main Methods:

  • Review of recent findings on alternative splicing.
  • Analysis of the interplay between transcription and splicing.

Related Experiment Videos

  • Investigation of promoter effects and RNA polymerase II elongation in AS regulation.

    • Main Results:

    • Alternative splicing affects 60% of human genes, contributing to proteomic complexity.
    • Transcription significantly impacts AS through promoter identity and occupation.
    • Factors with dual transcription-splicing functions and control of RNA polymerase II elongation are key mediators.

    Conclusions:

    • Alternative splicing is a fundamental process in gene expression, not an exception.
    • The coupling of transcription and splicing is critical for regulating gene output.
    • Understanding these mechanisms offers insights into human disease and proteomic diversity.