Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

N-H...S hydrogen bonds in a ferredoxin model.

Marc A Walters1, Cara L Roche, Arnold L Rheingold

  • 1Department of Chemistry, New York University, New York, New York 10003, USA. marc.walters@nyu.edu

Inorganic Chemistry
|May 24, 2005
PubMed
Summary

Researchers synthesized a novel iron-sulfur cluster complex that mimics the ferredoxin active site. This synthetic ferredoxin model achieves N-H...S hydrogen bonding, offering new insights into iron-sulfur cluster function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heterotrimetallic {LnOVPt} complexes with antiferromagnetic Ln-V coupling and magnetic memory.

Chemical communications (Cambridge, England)·2020
Same author

Aqueous Stability and Ligand Substitution of a Layered Cu(I)/Isocyanide-Based Organometallic Network Material with a Well-Defined Channel Structure.

Inorganic chemistry·2020
Same author

Fluorous Soluble Cyanine Dyes for Visualizing Perfluorocarbons in Living Systems.

Journal of the American Chemical Society·2020
Same author

Diastereoselective Synthesis of P-Stereogenic Secondary Phosphine Oxides (SPOs) Bearing a Chiral Substituent by Ring Opening of (+)-Limonene Oxide with Primary Phosphido Nucleophiles.

The Journal of organic chemistry·2020
Same author

Luminescence of Lanthanide Complexes with Perfluorinated Alkoxide Ligands.

Inorganic chemistry·2020
Same author

Synthesis of Anhydroryanodol.

Journal of the American Chemical Society·2020

Area of Science:

  • Bioinorganic Chemistry
  • Biomimetic Chemistry
  • Coordination Chemistry

Background:

  • Ferredoxins (Fd) are crucial iron-sulfur proteins involved in electron transfer.
  • Understanding the structure-function relationship of Fd active sites is vital.
  • Synthetic models aid in dissecting the roles of specific interactions, like hydrogen bonds.

Purpose of the Study:

  • To synthesize a novel iron-sulfur cluster complex that replicates key features of the ferredoxin active site.
  • To investigate the role of N-H...S hydrogen bonds in stabilizing and influencing the iron-sulfur cluster.
  • To characterize the structural and electronic properties of the synthetic model.

Main Methods:

  • Synthesis of the Fe4S4 complex {(CH3)3NCH2CONH2}2[Fe4S4((tBuS)4].

Related Experiment Videos

  • X-ray crystallography to determine the precise three-dimensional structure.
  • Spectroscopic and electrochemical methods for characterization (implied).

    • Main Results:

    • The synthetic complex successfully incorporated N-H...S hydrogen bonds, mimicking ferredoxin's polypeptide backbone interactions.
    • X-ray data revealed an unprecedented tetragonally elongated [Fe4S4]2+ cluster core, contrasting with ferredoxin's compressed core.
    • This represents the first synthetic ferredoxin model achieving direct N-H...S hydrogen bonding to the cluster.

    Conclusions:

    • The synthetic model demonstrates the feasibility of creating functional ferredoxin mimics with specific hydrogen-bonding interactions.
    • The observed elongated cluster core suggests alternative structural motifs are possible in iron-sulfur clusters.
    • This work provides a foundation for designing more sophisticated biomimetic iron-sulfur complexes.