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General, high-affinity approach for the synthesis of fluorophore appended protein nanoparticle assemblies

Marinella G Sandros1, De Gao, Cagil Gokdemir

  • 1Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.

Chemical Communications (Cambridge, England)
|June 2, 2005
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...