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First example of Rh:-32,-46 red cell phenotype
J R Storry1, M Gorman, N I Maddox
1National Reference Laboratoy for Blood Group Serology, American Red Cross Blood Services, 15601 Crabbs Branch Way, Rockville, MD 20855, USA.
Immunohematology
|December 1, 1994
Summary
This study identifies a novel red blood cell (RBC) phenotype, Rh:-32,-46, in a white male donor. This finding is significant as it
Area of Science:
- Immunology
- Genetics
- Hematology
Background:
- The Rh blood group system is complex, with numerous antigens and phenotypes.
- Rare Rh phenotypes, such as Rhnull and deletion types, are clinically significant.
- The Rh46 antigen has been previously associated with deletion types or Rh:32.
Purpose of the Study:
- To characterize a novel red blood cell (RBC) phenotype, Rh:-32,-46.
- To investigate the serological properties of the alloantibody found in the donor's serum.
- To compare this finding with previously described Rh:-46 RBCs.
Main Methods:
- Standard blood group typing techniques were employed.
- Serological testing included reactivity with various RBCs and enzyme/chemical treatments (ficin, papain, AET, DTT).
- Phenotyping of multitransfused patients was also assessed.
Main Results:
- A white male donor presented with a rare Rh:-32,-46 RBC phenotype, not associated with known Rh deletion or Rhnull types.
- The donor's serum contained an alloantibody reactive with most RBCs, except Rhnull and D-- types.
- Reactivity was enhanced by ficin/papain treatment and unaffected by AET/DTT.
Conclusions:
- This is the first report of an Rh:-32,-46 phenotype not linked to rare Rh deletion or Rhnull types.
- The findings expand the understanding of Rh antigen diversity and alloantibody formation.
- The rare Rh:w5,w19,-31,-34 phenotype observed in the donor is also noteworthy in Caucasian populations.