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Resolution of discrepant typings observed in paternity testing
1Community Blood Center, 349 South Main Street, Dayton, OH 45402, USA.
Immunohematology
|January 1, 1989
Summary
Contaminated typing reagents caused inaccurate red blood cell (RBC) phenotyping in a paternity test. Using multiple reagent sources is crucial for reliable paternity testing and accurate blood group analysis.
Area of Science:
- Immunogenetics
- Hematology
Background:
- Accurate red blood cell (RBC) phenotyping is essential for genetic testing, including paternity analysis.
- Discrepancies in blood group typing can arise from various factors, including reagent quality.
Purpose of the Study:
- To investigate the cause of discrepant red blood cell (RBC) phenotyping results in a paternity testing case.
- To highlight the importance of reagent quality control in genetic and paternity testing.
Main Methods:
- Red blood cell (RBC) phenotyping was performed using standard serological methods.
- Typing reagents from different sources were analyzed for potential contaminants.
- Immunogenetic analysis was conducted to identify the source of the discrepancy.
Main Results:
- Discrepant RBC phenotyping results between a child and alleged parents were identified.
- A contaminating antibody, anti-Bgb (HLA B-17), was detected in the anti-C and -Cw typing reagents.
- The presence of the contaminant antibody led to false-positive reactions, causing the initial discrepancies.
Conclusions:
- Contamination in serological typing reagents can lead to erroneous results in paternity testing.
- The use of reagents from multiple, independent sources is critical to ensure the accuracy and reliability of paternity testing.
- This case underscores the need for stringent quality control measures in the manufacturing and sử dụng of blood grouping reagents.