Related Experiment Video
Updated: Aug 17, 2026

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Published on: October 10, 2020
The expression and function of Sgt1 protein in eukaryotic cells
Magdalena Spiechowicz1, Anna Filipek
1Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur St., 02-093 Warsaw, Poland. m.spiechowicz@nencki.gov.pl.
Abstract:
The Sgt1 protein was discovered in yeast but later it was found in other eucaryotic organisms, including plants and mammals. It was suggested that the Sgt1 protein plays a role in the CBF3 kinetochore and the SCF ubiquitin ligase complexes. Since the distribution, properties and functions of the mammalian Sgt1 have not been extensively studied, we examined the expression of Sgt1 protein in the rat tissues and here we report the preliminary results. Using northern and western blots we found that the Sgt1 protein is expressed in various tissues and that brain, skeletal muscles and spleen contain high levels of this protein and its mRNA. Then, by western blot, we checked the localization of Sgt1 in some areas of the rat brain such as cortex, cerebellum and hippocampus and we found that cerebellum and cortex contain highest amounts of this protein. Using immunohistochemistry we found that the Sgt1 protein is present in both neurons and glial cells.
Related Concept Videos
Activation and Inactivation of G Proteins
Bacterial Translocation and Protein Secretion
Post-translational Translocation of Proteins to the RER
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
GTPases and their Regulation
Large G-proteins, also known...
Tail-anchoring of Proteins in the ER Membrane
Directing Proteins to the Rough Endoplasmic Reticulum

