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Updated: Aug 17, 2026

Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
[Sequencing and bioinformatic analysis of NRDRiso cDNA]
Jing Du1, Ge-Fei Liu, Gui-Ling Wang
1Department of Biochemistry, Shenyang Medical College, Shenyang, 110034, China. Dujing1106@sohu.com
Abstract:
This study describes the cDNA sequencing and the bioinformatic analysis of a novel NADP(H)-dependent retinol dehydrogenase/reductases isoform (NRDRiso). Based upon the concensus sequences of human and mouse NRDR coding region, we have identified a short 377 bp RT-PCR product from human liver tissue. The cDNA sequence of a NRDR isoform was then isolated using RACE approach and its sequence was analysed. The full-length cDNA is 1,003bp in length and was submitted to GenBank as NADP-dependent retinol dehydrogenase/reductase short isoform (NRDRiso). The open reading frames of NRDRiso cDNA is 525 bp.
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