Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipid transfer proteins from fruit: cloning, expression and quantification.

Laurian Zuidmeer1, W Astrid van Leeuwen, Ilona Kleine Budde

  • 1Department of Immunopathology, Sanquin, Amsterdam, The Netherlands.

International Archives of Allergy and Immunology
|June 23, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determinants of Poor Health-Related Quality of Life in Patients With Food Allergy.

Allergy·2026
Same author

Knowledge of chronic spontaneous urticaria management among Italian general practitioners: Baseline findings from the BRIDGE study.

The World Allergy Organization journal·2026
Same author

<i>In silico</i> prediction and IgE serum screening for potential allergenicity of novel foods-a case of fungal biomass Fermotein®.

Frontiers in allergy·2026
Same author

D-Dimer Elevation in Spontaneous Urticaria and Bullous Pemphigoid, Two Chronic Inflammatory Skin Diseases.

American journal of hematology·2026
Same author

IgE to cyclophilin Bet v 7 triggers mast cell activation and mediates cross-reactivity with Ara h 18 in children with seasonal allergic rhinitis.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026
Same author

BRIDGE implementation science study to improve chronic spontaneous urticaria outcomes in Italy.

The journal of allergy and clinical immunology. Global·2026
Same journal

Differences in Reported Anaphylaxis Associated With Common Nonsteroidal Anti-inflammatory Drugs: A Pharmacovigilance Study.

International archives of allergy and immunology·2026
Same journal

Association of Serum Galectin-3 Binding Protein with Acute Exacerbation, Inflammation, and Airway Remodeling in Pediatric Asthma.

International archives of allergy and immunology·2026
Same journal

Effectiveness of a Provincial Law to Improve Epinephrine Use for Anaphylaxis in Schools in Alberta, Canada: A Pre-Post Study.

International archives of allergy and immunology·2026
Same journal

Rab27 a regulates dendritic cell immune tolerogenic capacity.

International archives of allergy and immunology·2026
Same journal

Burden of Hereditary Angioedema in Adults: Impact of Disease and Patient Characteristics.

International archives of allergy and immunology·2026
Same journal

Predictors of Treatment Response to Omalizumab in Children with Chronic Spontaneous Urticaria: A Real World Study.

International archives of allergy and immunology·2026
See all related articles

Recombinant apple lipid transfer protein (LTP), Mal d 3, was successfully cloned and expressed. This allergen is valuable for diagnosing food allergies and improving traceability of dangerous LTPs.

Area of Science:

  • Food allergy research
  • Allergen characterization
  • Biotechnology

Background:

  • Lipid transfer proteins (LTPs) are stable, potentially life-threatening food allergens.
  • Apple LTP (Mal d 3) and peach LTP (Pru p 3) are significant allergens.

Purpose of the Study:

  • To clone and express recombinant apple LTP (Mal d 3).
  • To develop quantitative tests for measuring fruit LTPs.
  • To compare the immune reactivity of recombinant and natural Mal d 3.

Main Methods:

  • Cloning and expression of Mal d 3 and Pru p 3 cDNA in Pichia pastoris.
  • Purification of recombinant proteins using cation exchange chromatography.
  • Immune reactivity testing including RAST inhibition, immunoblotting, and basophil histamine release.

Related Experiment Videos

Main Results:

  • Recombinant Mal d 3 (rMal d 3) demonstrated IgE-binding potency and biological activity similar to natural Mal d 3 (nMal d 3).
  • Developed a sandwich ELISA for apple LTP and a cross-reactive assay for other fruit/nut LTPs.
  • Developed a competitive RIA using polyclonal antibodies.

Conclusions:

  • Recombinant Mal d 3 is a useful tool for component-resolved diagnosis of food allergy.
  • Developed assays enhance the traceability of potentially dangerous LTP allergens.