Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue factor as a link between wounding and tissue repair.

Jiang Chen1, Michael Kasper, Tobias Heck

  • 1University of Heidelberg, Department of Medicine I, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.

Diabetes
|June 29, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prioritizing Conservation of Trailing-Edge Populations for Future Climate-Resilient Forests.

Global change biology·2026
Same author

White spruce (Picea glauca) population differences in needle anatomy, foliar water uptake and aquaporin expression indicate trade-offs between hydraulic safety and productivity.

Tree physiology·2025
Same author

A high-resolution database of historical and future climate for Africa developed with deep neural networks.

Scientific data·2025
Same author

Short- and Long-Term Growth Response to Multiple Drought Episodes: Evidence of Genetic Adaptation in a Conifer Species.

Ecology and evolution·2025
Same author

Validation of global precipitation time series products against tree ring records and remotely sensed vegetation greenness.

PloS one·2024
Same author

ClimateEU, scale-free climate normals, historical time series, and future projections for Europe.

Scientific data·2020

Diabetic wound repair is slowed by low tissue factor (TF) levels. Enhancing TF in diabetic mouse wounds accelerated healing by promoting new blood vessel growth and increasing blood flow.

Area of Science:

  • Wound healing research
  • Diabetic complications
  • Vascular biology

Background:

  • Wound repair initiates with inflammation, tissue factor (TF) induction, fibrin formation, and new blood vessel growth.
  • In diabetes, TF induction after wounding is reduced, impacting subsequent vascular endothelial growth factor (VEGF) and alpha-smooth muscle actin (alpha-SMA) expression.
  • This diminished TF response contributes to delayed healing in diabetic individuals.

Purpose of the Study:

  • To investigate the role of TF deficiency in impaired diabetic wound healing.
  • To determine if enhancing TF levels can improve wound repair in diabetic models.
  • To establish TF as a critical link between inflammation and repair processes.

Main Methods:

  • Somatic TF gene transfer was used to increase TF expression in cutaneous wounds of diabetic mice.

Related Experiment Videos

  • Vascular endothelial growth factor (VEGF) transcription and translation were measured.
  • New blood vessel formation (angiogenesis) and wound closure rates were assessed.

    • Main Results:

    • Enhanced TF expression significantly increased VEGF transcription and translation.
    • TF gene transfer promoted the formation of new blood vessels and elevated blood flow in diabetic wounds.
    • The time for 50% wound closure decreased from 5.5 days in untreated diabetic mice to 4.1 days in TF-treated mice.
    • TF-treated diabetic mice showed wound closure times comparable to non-diabetic controls.

    Conclusions:

    • Diabetic cutaneous wounds exhibit a relative TF deficiency compared to non-diabetic wounds, contributing to delayed repair.
    • Increasing TF levels can effectively enhance diabetic wound healing by promoting angiogenesis and improving blood flow.
    • TF is a key mediator connecting the early inflammatory response to subsequent reparative processes in wound healing.