Related Experiment Video

Updated: Aug 12, 2026

Absolute Quantum Yield Measurement of Powder Samples
14:20

Absolute Quantum Yield Measurement of Powder Samples

Published on: May 12, 2012

How to improve quality assurance in fluorometry: fluorescence-inherent sources of error and suited fluorescence

U Resch-Genger1, K Hoffmann, W Nietfeld

  • 1Federal Institute for Materials Research and Testing, Working Group Optical Spectroscopy, Division I.3, BAM, Berlin, Germany, ute.resch@bam.de

Journal of Fluorescence
|June 30, 2005
PubMed

More Related Videos

Quantifying X-Ray Fluorescence Data Using MAPS14:58

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
Flame Photometry: Lab01:16

Flame Photometry: Lab

In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...