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[Screening the antibody mimic peptide binding to hepatocellular carcinoma cells by phage display technique]
Han-ping Li1, Yu-ling Zheng, Hong Zhou
1The State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Aim:
To screen the antibibody mimic peptide binding to hepatocellular carcinoma (HCC) from the circular 7-mer peptide libraries.
Methods:
The enrichment of phage was achieved by repetitious biopanning. The affinity of peptide was evaluated by ELISA, and the binding site of the peptide was analysed by the competition ELISA.
Results:
The output of phage gradually rose along with increasing of biopanning times. The obtained circular 7-mer peptide showed good binding activity to hepatocellular carcinoma cell lines SMMC-7721 and BEL-7402 (P<0.05), and its affinity to SMMC-7721 cells was superior to that of BEL-7402 (P<0.05). At the level of alpha=0.01, the screened 7-mer peptide monoclonal stocks could obviously compete with scFv to bind SMMC-7721 cells (0.005
Conclusion:
The peptide obtained through biopanning was specific and effective, which may provide further support for target therapy of HCC.
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