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Updated: Aug 17, 2026
Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
Published on: September 12, 2011
In vivo DNA gene electro-transfer: a systematic analysis of different electrical parameters
Immacolata Zampaglione1, Mirko Arcuri, Manuela Cappelletti
1Istituto di ricerche di Biologia Molecolare (IRBM), Via Pontina Km 30.600, 00040 Pomezia (Rome), Italy.
Background:
Intramuscular plasmid injection followed by electroporation is an efficient method for gene therapy or vaccination. Several protocols have been described that give good transduction levels with several reporter genes.
Methods:
In this work we have explored the efficiency of gene delivery upon variation of the different electrical parameters such as pulse length frequency and voltage monitoring both on short- and long-term protein production.
Results:
Having defined the best performing parameters, we have designed a short electric treatment that gives good levels of plasmid-encoded protein in different species such as mice, rabbits and monkeys.
