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Related Experiment Videos

[Drug analytics in oral fluid using immunoassay].

O Dierich1, M Soyka

  • 1Psychiatrische Klinik der Ludwig-Maximilian-Universität, München.

Fortschritte Der Neurologie-Psychiatrie
|July 14, 2005
PubMed
Summary

Drug testing in oral fluid shows limited accuracy compared to urine analysis for most substances. While methadone detection was comparable, opioids, barbiturates, cannabinoids, and benzodiazepines yielded unsatisfactory results, questioning its reliability.

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Area of Science:

  • Forensic Toxicology
  • Clinical Chemistry
  • Analytical Chemistry

Background:

  • Oral fluid drug analytics are relevant for clinical and forensic applications, including impaired driving assessments.
  • Limited studies exist on the validity and reliability of various oral fluid drug testing methods.
  • Establishing accurate cut-off levels is crucial for interpreting drug concentrations in oral fluid.

Purpose of the Study:

  • To evaluate the reliability of drug analytics in oral fluid using specific immunoassay tests (CEDIA and EMIT).
  • To compare the accuracy of oral fluid drug testing with traditional urine analysis.
  • To define cut-off levels for various drugs in oral fluid based on ROC curve analysis.

Main Methods:

  • Utilized CEDIA and EMIT tests for drug analysis in oral fluid samples.

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  • Analyzed 97 saliva and 103 urine samples from 31 opioid-dependent patients.
  • Employed Receiver Operating Characteristic (ROC) curves to determine substance-specific cut-off values.

    • Main Results:

    • Oral fluid methadone detection showed comparable results to urine analysis.
    • Results for most other drugs, including opioids and barbiturates, were less favorable in oral fluid.
    • Oral fluid analysis for cannabinoids and benzodiazepines yielded unsatisfactory accuracy.

    Conclusions:

    • Current oral fluid drug testing methods are less accurate than urine analysis for many drug classes.
    • Urine testing via immunoassays or gas chromatography remains the most reliable non-invasive method.
    • Oral fluid drug testing may serve as a supplementary tool in addiction treatment and for epidemiological road safety surveys.