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Updated: Aug 17, 2026

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
Published on: June 30, 2016
[The effect of macrophage colony stimulating factor on laryngeal cancer Hep-2 cell line]
Pingjiang Ge1, Baoquan Zhang, Jianmin Zhang
1Department of Otolaryngology-Head and Neck Surgery, Guangdong Province People's Hospital, Guangzhou, 510080, China.
Objective:
To investigate the possible role of M-CSF and its receptor in the laryngeal carcinoma Hep-2 cell line.
Method:
Cell proliferation was assayed by MTT analysis and [3H] thymidine incorporation, after Hep-2 cell line culturing with M-CSF and M-CSFR. M-CSF concentration of surpernatant measured by a ELISA technique. Immunohistochemistry was performed on Hep-2 cell line and laryngeal carcinoma tissue.
Result:
The concentration of M-CSF increased after cell culturing. M-CSF can reduced the Hep-2 cell count. The action can be blocked by Anti-M-CSFR.
Conclusion:
Our observations support the hypothesis that M-CSF may inhibit the growth and differentiation in Hep-2 cell line.
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