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Lactobacillus plantarum subsp. argentoratensis subsp. nov., isolated from vegetable matrices
Françoise Bringel1, Anna Castioni2, Daniel K Olukoya3
1Laboratoire de Dynamique, Evolution et Expression de Génomes de Microorganismes, Université Louis Pasteur/CNRS FRE 2326, 28 rue Goethe, 67083 Strasbourg, France.
Summary
A new subspecies, Lactobacillus plantarum subsp. argentoratensis, has been identified. This finding is based on genetic and phenotypic analysis of fourteen closely related Lactobacillus plantarum strains from vegetable sources.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- Lactobacillus plantarum is a common bacterium found in various food sources.
- Atypical PCR results were observed in some Lactobacillus plantarum strains from vegetables.
Purpose of the Study:
- To investigate the taxonomic status of Lactobacillus plantarum strains with atypical multiplex-PCR results.
- To characterize these strains using phylogenetic and phenotypic analyses.
Main Methods:
- Species-specific multiplex-PCR assay.
- Phylogenetic analysis of recA and cpn60 genes.
- Phenotypic and genomic trait evaluation.
Main Results:
- Fourteen strains showed an atypical amplification pattern with a species-specific multiplex-PCR.
- Phylogenetic and phenotypic data revealed a homogeneous group of closely related strains.
- The proposed new subspecies is named Lactobacillus plantarum subsp. argentoratensis.
Conclusions:
- The identified strains represent a distinct subspecies within Lactobacillus plantarum.
- Lactobacillus plantarum subsp. argentoratensis is proposed based on genetic and phenotypic evidence.
- This finding refines the taxonomy of the Lactobacillus plantarum species.