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Updated: Aug 16, 2026
Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay
Published on: December 29, 2023
[Screening for new binding proteins which interact with BM2 of influenza B virus with yeast two-hybrid system]
Hong Yu1, Li-hong Yao, Ai-jun Chen
1State Key Laboratory of Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
Objective:
To explore the role of BM2 protein in the life cycle of influenza B virus.
Methods:
The authors screened human kidney MATCHMAKER cDNA library for new binding partners of BM2 of influenza B virus by using the yeast two hybrid system with truncated BM2 (26-109 aa) as the bait.
Results:
Six positive plasmids encoding N-acetylneuraminate pyruvate lyase, angiopoietin 3, zinc finger protein 251, ribosomal protein S20, protein arginine N-methyltransferase 1 variant 1 (PRMT) and transcription factor-like 1 (TCFL1) were obtained.
Conclusion:
The results suggest that BM2 may play an important role in the life cycle of influenza B virus.
