Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phasing diffraction data from a stream of hydrated proteins.

Jin Song Wu1, John C H Spence

  • 1Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287-1504, USA. jinsong.wu@asu.edu

Journal of the Optical Society of America. A, Optics, Image Science, and Vision
|August 2, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unsupervised learning approaches to characterizing heterogeneous samples using X-ray single-particle imaging.

IUCrJ·2022
Same author

Co-flow injection for serial crystallography at X-ray free-electron lasers.

Journal of applied crystallography·2022
Same author

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography.

IUCrJ·2021
Same author

Reprint of: Inversion of dynamical Bragg intensities to complex structure factors by iterated projections. For Ultramic. 2020. ("Pico" Festschrift, May 2021).

Ultramicroscopy·2021
Same author

Early-stage dynamics of chloride ion-pumping rhodopsin revealed by a femtosecond X-ray laser.

Proceedings of the National Academy of Sciences of the United States of America·2021
Same author

Synchronous RNA conformational changes trigger ordered phase transitions in crystals.

Nature communications·2021
Same journal

Multi-module collaborative optimization-driven fast speckle correlation imaging in variable environments.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Secrecy performance analysis of NOMA-UWOC systems over a vertically stratified WGG oceanic turbulence channel.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Backscattering of plane waves in a composite system containing a rough surface and anisotropic scatterers.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Aspherical surface construction methods based on extended Jacobi polynomials.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

OCT sidelobe suppression method based on dual-path phase sinusoidal modulation and minimum value fusion.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Optical design concepts using wavelength-selective diffractive optics to enable miniaturized multimodal endoscopic imaging across separated spectral ranges.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
See all related articles

This study introduces a new iterative phasing method to determine the 3D structure of large molecules like proteins using X-ray diffraction patterns. The method successfully reconstructs molecular structures from simulated data of hydrated proteins in ice.

Area of Science:

  • Structural biology
  • X-ray crystallography
  • Biophysics

Background:

  • Determining the 3D structure of large molecules is crucial for understanding their function.
  • Continuous diffraction patterns from laser-aligned molecules in ice present unique phasing challenges.
  • Existing methods may struggle with the complexity of hydrated protein samples.

Purpose of the Study:

  • To develop and validate an iterative phasing method for continuous X-ray diffraction data.
  • To extract the common 3D structure of identical, aligned large molecules embedded in ice.
  • To address phase determination challenges in serial crystallography of hydrated biomolecules.

Main Methods:

  • Iterative phasing algorithm utilizing charge-flipping and hybrid input-output.

Related Experiment Videos

  • Analysis of continuous diffraction patterns from laser-aligned molecules in ice.
  • Application to simulated data of hydrated lysozyme proteins.

    • Main Results:

    • Successfully identified boundaries (supports) between protein, ice, and the outer iceball.
    • Reconstructed the common 3D structure from simulated diffraction data.
    • Demonstrated the algorithm's applicability to hydrated lysozyme and considered the effect of empty ice balls.

    Conclusions:

    • The developed iterative phasing method is effective for 3D structure determination from continuous diffraction patterns.
    • The approach can handle hydrated biomolecules and accounts for experimental factors like empty ice balls.
    • The algorithm offers a potential advancement for serial crystallography and molecular imaging.