Related Experiment Video
Updated: Jul 16, 2026
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
Published on: September 28, 2013
Quantitative analysis of membrane proteins from breast cancer cell lines BT474 and MCF7 using multistep solid phase
Yang Shi1, Rong Xiang, Csaba Horváth
1Department of Chemical Engineering, Yale University, New Haven, CT 06520-8286, USA.
Abstract:
We introduce a new multistep mass tagging technique and show its utility for reducing sample complexity when coupled with two-dimensional liquid chromatography/nano-electrospray ionization ion trap mass spectrometry (2D LC/nano ESI-MS). Solid-phase mass tagging reagents were used to identify and obtain relative quantitation of membrane proteins from two established breast cancer cell lines, BT474 and MCF7. The results presented in this study show that sample complexity can be reduced with corresponding increases in protein identification and quantitation.
