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Related Experiment Videos

Intragenic tandem repeats generate functional variability.

Kevin J Verstrepen1, An Jansen, Fran Lewitter

  • 1Whitehead Institute for Biomedical Research/Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA.

Nature Genetics
|August 9, 2005
PubMed
Summary
This summary is machine-generated.

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Intragenic DNA repeats in yeast genes, particularly those encoding cell-wall proteins, drive genetic variation. This variation enhances fungal adaptation and immune evasion through diverse cell surface antigens.

Area of Science:

  • Genomics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Tandemly repeated DNA sequences are dynamic genomic elements, often found in intergenic regions.
  • Intragenic triplet repeat expansions in humans are linked to diseases like Huntington's chorea and fragile X syndrome.
  • The evolutionary persistence of intragenic repeats suggests potential adaptive advantages.

Purpose of the Study:

  • To investigate the functional role and evolutionary significance of intragenic repeats in Saccharomyces cerevisiae.
  • To explore the link between intragenic repeats and cell-wall protein genes in yeast.
  • To understand how repeat-induced gene size variation contributes to phenotypic diversity and adaptation.

Main Methods:

  • Genomic analysis of Saccharomyces cerevisiae to identify genes containing intragenic repeats.

Related Experiment Videos

  • Investigation of recombination events associated with these repeats.
  • Phenotypic analysis of yeast strains with varying intragenic repeat numbers, focusing on cell-wall related traits.

    • Main Results:

    • Most genes with intragenic repeats in Saccharomyces cerevisiae encode cell-wall proteins.
    • Intragenic repeats promote frequent recombination, leading to gene size expansion and contraction.
    • This size variation quantitatively alters phenotypes such as adhesion, flocculation, and biofilm formation.

    Conclusions:

    • Intragenic repeat variation in yeast cell-wall protein genes generates functional diversity in cell surface antigens.
    • This diversity facilitates rapid adaptation to environmental changes.
    • Such variation may enable pathogens, including fungi, to evade host immune systems.