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Updated: Aug 16, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
Interferon gene regulation: not all roads lead to Tolls
Caroline A Jefferies1, Katherine A Fitzgerald
1Department of Biochemistry and Biotechnology Institute, Trinity College Dublin, Ireland.
Abstract:
Many infectious agents elicit a type I interferon response but, until recently, the molecular details that coordinate interferon (IFN)-alpha and -beta expression during infection were unknown. Innate immune pattern recognition receptors, including Toll-like receptors and cytoplasmic RNA helicases such as retinoic acid-inducible gene, that sense viral nucleic acids have been discovered. Using distinct mechanisms, these receptors trigger cellular signaling pathways, culminating in the activation of interferon regulatory factors that transcriptionally induce IFN-alpha and IFN-beta genes.
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