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Updated: Jul 11, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Published on: January 6, 2015
Pre-unfolding resonant oscillations of single green fluorescent protein molecules
Giancarlo Baldini1, Fabio Cannone, Giuseppe Chirico
1Laboratory for Advanced BioSpectroscopy, Physics Department, and Centro Nazionale delle Ricerche (CNR)-Istituto Nazionale per la Fisica della Materia (INFM), University of Milano-Bicocca, Milano I-20126, Italy. baldini@mib.infn.it
Abstract:
Fluorescence spectroscopy of a green fluorescent protein mutant at single-molecule resolution has revealed a remarkable oscillatory behavior that can also be driven by applied fields. We show that immediately before unfolding, several periodic oscillations among the chemical substates of the protein chromophore occur. We also show that applied alternating electric or acoustic fields, when tuned to the protein characteristic frequencies, give rise to strong resonance effects.

