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Updated: Aug 16, 2026

The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation
Published on: August 21, 2012
Altered muscarinic receptor subtype expression and functional responses in cyclophosphamide induced cystitis in rats
1Department of Pharmacology, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Abstract:
In the in vitro study, it was investigated whether the expression of muscarinic receptors and cholinergic responses were altered in the situation of experimental cystitis. Rats were treated with cyclophosphamide intraperitoneally and the bladders were excised 36-100 h later. Immunohistochemistry and immunoblotting showed all subtypes of the muscarinic receptor (M1-M5) to be present in the specimens from inflamed urinary bladders and controls. In the cyclophosphamide-treated rats, the expression of muscarinic M5 receptors was increased by more than 40 times (p<0.01; n=8) both in the smooth muscle and the urothelium. Both the maximal contractile response to carbachol and to a high potassium concentration was approximately halved in cyclophosphamide-treated tissues, whereas the reduction was substantially greater in response to low carbachol concentrations (
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