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Published on: April 7, 2008
[Construction of co-expression vector pSLC-IRES-IL-2 and its expression in COS-7 cells]
Li Hou1, Yue-ran Zhao, Lai-cheng Wang
1Medical Research Center of Shandong Provincial Hospital, Shandong University, Jinan 250021, China. angelhl@126.com
Aim:
To construct an eukaryotic co-expression plasmid pSLC-IRES-IL-2, and to express it in COS-7 cells.
Methods:
Human IL-2 and SLC genes were cloned by RT-PCR and PCR, respectively, and then the eukaryotic expression plasmid pSLC-IRES-IL-2 was constructed.The constructed plasmid was transfected into COS-7 cells by electroporation method. The expression of SLC and IL-2 was detected by Western blot.
Results:
SLC and IL-2 in the culture supernatant and lysate of transfected COS-7 cells were detected by Western blot. The relative molecular masses of the expressed products were consistent with the theoretical values.
Conclusion:
The SLC and IL-2 co-expression plasmid is successfully constructed and expressed in COS-7 cells, which offers a pathway for research on gene therapy of tumors.
