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Updated: Aug 16, 2026
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Published on: August 22, 2019
[A specific human scFv against malignant melanoma selected from a large human phage antibody library]
Miao Li1, Yu-ping Chen, Gang Wang
1Department of Dermatology Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. youlonglm@sina.com
Aim:
To obtain the specific human scFv against malignant melanoma (MM) using phage antibody library technology.
Methods:
The library was panned with a certain kind of MM cells for 4 rounds. The antigen binding activities of random clones were tested by ELISA in order to select specific antibodies, which were then examined by DNA sequence analysis and immunohistochemistry.
Results:
In ELISA and immunohistochemical staining, the positive clone selected from the 80 random clones was able to bind one kind of MM cell specifically, while not able to bind other irrelavant cells or antigens, such as human's squamous carcinoma cells, keratinocytes, melanocytes, keratin, trypsinase, transferrin and mouse IgG, etc. The antibody's V(H) genes belonged to human IgG V(H)5 subgroup and the V(L) belonged to human V(kappa) subtype determined by DNA sequence analysis.
Conclusion:
One specific human phage antibody against MM cells was obtained by phage display technique, which may be useful for further development of tumor-targeted therapies.
