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Updated: Aug 16, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
Published on: November 29, 2024
[The influence of platelets on PTA1 expression and PDGF release from ECV304 cells]
Chang-ling Li1, Rui-huan Xu, Hong-de Zhang
1Department of Laboratory Diagnosis, Punan Hospital, Shanghai 200125, China. Lchl12@yahoo.com
Aim:
To study the influence of platelets on the expression of platelet/T cell activation antegen 1 (PTA1) and release of platelet-derived growth factor (PDGF) from vascular endothelial cells (ECV304) stimulated with pregnancy induced hypertention (PIH) patient sera.
Methods:
The expression of PTA1 on ECV304 cells stimulated with PIH patient sera and incubated with or without platelets was analyzed by flow cytometry. ELISA was employed to measure the quantity of PDGF released from ECV304 cells.
Results:
After incubation with platelets, PTA1 expression on ECV304 cells stimulated with PIH patient sera decreased. PIH sera induced more PDGF release from ECV304 cells than sera from normal pregnant women. Coculture with platelets increased PDGF release from ECV304 stimulated with PIH patient sera.
Conclusion:
PTA1 molecule may mediate the adhesion between ECV304 cells and platelets, which lead to the release of PDGF.
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