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Published on: August 21, 2019
[Development and application of a method for rapid quantification of recombinant ICOS-mIg fusion protein]
1Central Laboratory of Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Aim:
To set up a double antibody sandwich ELISA for rapid quantification of recombinant ICOS-mIg fusion protein and evaluate its sensitivity, specificity and linear detection range.
Methods:
Recombinant ICOS-mIg fusion protein was prepared by molecular biology technique. Sheep anti-mouse IgG and horse anti-mouse IgG-HRP conjugate were selected as coating antibody and detection antibody, respectively for sandwich ELISA by pair-matching experiments. A standard curve for determining the recombinant ICOS-mIg fusion protein was plotted and applied for quantification of ICOS-mIg fusion protein.
Results:
A sandwich ELISA for quantification of the recombinant ICOS-mIg fusion protein was developed. Its linear detection was 7.8 to 500 microg/L(R(2)=0.9911, P<0.0001).
Conclusion:
The recombinant ICOS-mIg fusion protein can be determined by this sandwich ELISA rapidly and precisely.
