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Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
07:20

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR

Published on: February 4, 2018

[Genetic characterization of pathogenic Borrelia, group A14S, isolated in Ukraine]

V V Nefedova, E I Korenberg, Iu V Andreĭchuk

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |September 9, 2005
    PubMed

    Insights

    A new Borrelia bacterium, Borrelia Ir-5215, was identified in Ukraine. This tick-borne pathogen belongs to the Borrelia burgdorferi sensu lato group A14S, found in Central Europe and southern Ukraine.

    Area of Science:

    • Microbiology
    • Genetics
    • Epidemiology

    Context:

    • Ticks (Ixodes ricinus) in Ukraine harbor diverse Borrelia species.
    • Understanding Borrelia diversity is crucial for public health and disease surveillance.

    Purpose:

    • To identify and characterize a novel Borrelia isolate from Ukrainian ticks.
    • To determine the genetic relatedness of the isolate to known Borrelia burgdorferi sensu lato species.

    Summary:

    • Borrelia Ir-5215, isolated from Ixodes ricinus ticks in Ukraine, was analyzed using rRNA spacer region polymorphism and gene sequencing.
    • Genetic analysis revealed significant differences from six European Borrelia burgdorferi sensu lato species but high similarity (>95.1% for 5S-23S rRNA, 99.4% for 16S rRNA) to genome group A14S representatives.
    • The findings suggest Borrelia Ir-5215 is a new member of the pathogenic Borrelia burgdorferi sensu lato genome group A14S, extending its known geographical range.

    Impact:

    • Identifies a novel Borrelia strain, expanding the known genetic diversity of this Lyme disease agent.
    • Suggests the geographical spread of Borrelia genome group A14S into southern Ukraine, impacting regional disease risk assessment.
    • Highlights the importance of ongoing tick-borne pathogen surveillance in emerging regions.

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