Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T cell function detected in murine bone marrow cells.

M Harada, T Takiguchi, K Hattori

    The Japanese Journal of Experimental Medicine
    |February 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Association of p53 expression with second primary tumour development in hypopharyngeal carcinoma.

    The Journal of laryngology and otology·2000
    Same author

    [Successful aortic root replacement for prosthetic valve endocarditis using the freestyle stentless bioprosthesis: report of two cases].

    Kyobu geka. The Japanese journal of thoracic surgery·2000
    Same author

    [New combination chemotherapy in urological cancers].

    Gan to kagaku ryoho. Cancer & chemotherapy·2000
    Same author

    Effect of adaptive plasticity of linear vestibulo-ocular reflex upon angular vestibulo-ocular reflex.

    Auris, nasus, larynx·2000
    Same author

    Heparin-coated circuits prevent renal dysfunction after open heart surgery.

    Osaka city medical journal·2000
    Same author

    Characterization of XET-related genes of rice.

    Plant physiology·2000
    Same journal

    Protein extractabilities from the erythrocyte membranes in patients with chorea-acanthocytosis.

    The Japanese journal of experimental medicine·1990
    Same journal

    Estrogen and progesterone receptor levels in 7,12-dimethylbenz[a]anthracene-induced rat mammary tumors in each week--check points about experimental studies using DMBA-induced tumors.

    The Japanese journal of experimental medicine·1990
    Same journal

    Aggregated distribution of infective spots composed of Leptotrombidium pallidum, highly prevalent with Rickettsia tsutsugamushi, demonstrated by sentinel voles, Microtus montebelli, on the ground.

    The Japanese journal of experimental medicine·1990
    Same journal

    Suppression of footpad reaction in mice by anthralin.

    The Japanese journal of experimental medicine·1990
    Same journal

    Characterization and change of phospholipids in the aorta of Watanabe hereditable hyperlipidemic rabbit.

    The Japanese journal of experimental medicine·1990
    Same journal

    A new technique of in vitro assay of antifilarials using different life-forms of Acanthocheilonema viteae.

    The Japanese journal of experimental medicine·1990
    See all related articles

    Researchers separated immunocompetent T cells from hematopoietic stem cells in mouse bone marrow. This separation method could help prevent graft-versus-host disease in human bone marrow transplants.

    Area of Science:

    • Immunology
    • Hematology
    • Cell Biology

    Background:

    • Bone marrow contains both immunocompetent lymphocytes and hematopoietic stem cells.
    • Distinguishing and separating these cell populations is crucial for understanding immune responses and for therapeutic applications like bone marrow transplantation.

    Purpose of the Study:

    • To investigate the separation of T cell populations with graft-versus-host reaction (GVHR) capabilities from hematopoietic stem cells using BSA discontinuous density gradient centrifugation.
    • To assess the correlation between theta antigen expression and T cell function in mature lymphocytes.

    Main Methods:

    • Fractionation of mouse bone marrow cells using BSA discontinuous density gradient centrifugation.
    • In vitro assessment of lymphocyte response to T cell mitogens and alloantigens.

    Related Experiment Videos

  • Evaluation of graft-versus-host reaction (GVHR) using spleen weight assay and inhibition of erythroid cell growth.
  • Quantification of theta antigen expression via cytotoxic and absorption tests.
  • Determination of hemopoietic stem cells by spleen colony formation assay.

    • Main Results:

    • A lymphocyte-rich fraction with high density exhibited T cell functions, including response to mitogens/alloantigens and ability to mount GVHR.
    • This fraction showed low theta antigen expression, suggesting mature T cells may have reduced antigen levels.
    • Hematopoietic stem cells peaked in colony-forming efficiency at low density.
    • Successful separation of GVHR-causing immunocompetent cells from hematopoietic stem cells was achieved.

    Conclusions:

    • Mouse bone marrow fractionation effectively separates T cells with GVHR potential from hematopoietic stem cells.
    • Theta antigen levels may not directly correlate with T cell function in postthymic mature lymphocytes.
    • This separation technique holds promise for reducing GVHR in allogeneic bone marrow transplantation in humans.