Related Experiment Videos
Genome-wide responses to DNA-damaging agents
Rebecca C Fry1, Thomas J Begley, Leona D Samson
1Biological Engineering Division and Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. rfry@mit.edu
Annual Review of Microbiology
|September 13, 2005
Summary
Eukaryotic cells activate complex networks to protect against DNA damage. These responses involve expected functions like DNA repair and unexpected roles in protein trafficking and degradation.
Area of Science:
- Molecular Biology
- Cell Biology
- Genomics
Background:
- Eukaryotic cells exhibit dynamic responses to DNA-damaging agents.
- Genome-wide studies reveal intricate regulatory networks controlling these responses.
- Understanding these networks is crucial for comprehending cellular resilience.
Purpose of the Study:
- To review genome-wide studies on cellular responses to DNA damage.
- To integrate diverse datasets for a systems-level view of damage response.
- To highlight both expected and unexpected cellular functions involved.
Main Methods:
- Analysis of genome-wide mRNA regulation studies.
- Integration of protein-protein and protein-DNA interaction data.
- Incorporation of subcellular localization information.
Main Results:
- Identification of networked cellular responses to DNA damage.
- Discovery of unexpected roles for proteins in cellular protection.
- Demonstration of a multifaceted cellular defense system.
Conclusions:
- Cellular protection from DNA damage involves a complex protein network.
- This network spans diverse cellular functions beyond DNA repair.
- A systems biology approach provides comprehensive insights into damage response.