Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein microarray-based screening of antibody specificity.

Rhonda Bangham1, Gregory A Michaud, Barry Schweitzer

  • 1Research and Development, Protometrix Inc., Branford, CT, USA.

Methods in Molecular Medicine
|September 15, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of molecular glues that bind FKBP12 and structurally distinct targets using DNA-encoded libraries.

Nature communications·2026
Same author

A small-molecule VHL molecular glue degrader for cysteine dioxygenase 1.

Nature chemical biology·2025
Same author

Activation of human STING by a molecular glue-like compound.

Nature chemical biology·2023
Same author

A small-molecule inhibitor of C5 complement protein.

Nature chemical biology·2019
Same author

Corrigendum: SMN2 splice modulators enhance U1-pre-mRNA association and rescue SMA mice.

Nature chemical biology·2016
Same author

Corrigendum: SMN2 splice modulators enhance U1-pre-mRNA association and rescue SMA mice.

Nature chemical biology·2015
Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015
Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014
Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014
Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014
Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014
Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014
See all related articles

Developing specific antibodies is crucial for therapeutics and high-throughput analyses. Protein arrays enable simultaneous screening of thousands of proteins, offering a powerful method for testing antibody specificity and improving research accuracy.

Area of Science:

  • Biotechnology
  • Immunology
  • Proteomics

Background:

  • Increasing demand for specific antibodies in therapeutics and high-throughput protein analysis.
  • Need for robust methods to assess antibody specificity against a wide range of targets.

Purpose of the Study:

  • To describe methods for probing protein arrays with antibodies.
  • To highlight technical considerations for antibody specificity profiling using protein arrays.

Main Methods:

  • Utilizing yeast proteome arrays for antibody specificity profiling.
  • Simultaneous screening of thousands of proteins in normalized quantities.
  • Detailed description of antibody probing techniques for protein arrays.

Main Results:

Related Experiment Videos

  • Demonstrated the utility of yeast proteome arrays for antibody specificity assessment.
  • Provided insights into technical challenges and solutions for antibody-protein interaction studies.

Conclusions:

  • Protein arrays are an effective tool for evaluating antibody specificity.
  • Standardized methods are essential for reliable antibody characterization in proteomic applications.