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A study on ceftriaxone-induced lipid peroxidation using 4-hydroxy-2-nonenal as model marker
Santanu Chakraborty1, Pritesh Dev Bhuti, Supratim Ray
1Division of Medicinal and Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032 India.
Acta Poloniae Pharmaceutica
|September 16, 2005
Summary
Ceftriaxone significantly induces lipid peroxidation in goat liver. Ascorbic acid effectively inhibits this ceftriaxone-induced lipid peroxidation, confirming its protective role.
Area of Science:
- Biochemistry
- Toxicology
- Pharmacology
Background:
- Lipid peroxidation is a key indicator of cellular damage.
- Ceftriaxone is a widely used antibiotic with potential side effects.
- 4-Hydroxy-2-nonenal (4-HNE) is a reliable biomarker for lipid peroxidation.
Purpose of the Study:
- To investigate the effect of ceftriaxone on lipid peroxidation in goat liver homogenate.
- To evaluate the potential protective role of ascorbic acid against ceftriaxone-induced lipid peroxidation.
Main Methods:
- Goat liver homogenate was used as an in vitro model.
- 4-Hydroxy-2-nonenal (4-HNE) was employed as the marker for lipid peroxidation.
- The impact of ceftriaxone exposure and ascorbic acid co-administration was assessed.
Main Results:
- Ceftriaxone significantly increased levels of 4-HNE, indicating induced lipid peroxidation.
- Ascorbic acid demonstrated a significant inhibitory effect on ceftriaxone-induced lipid peroxidation.
- These findings align with previous studies using malondialdehyde as a marker.
Conclusions:
- Ceftriaxone induces significant lipid peroxidation in goat liver tissue.
- Ascorbic acid exhibits a protective effect against ceftriaxone-induced oxidative stress.
- 4-HNE serves as a valid marker for assessing drug-induced lipid peroxidation.