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Updated: Aug 16, 2026
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Evaluation of ascorbic acid as suppressor of cyclophosphamide induced lipid peroxidation using common laboratory
Supratim Ray1, Chandana Sengupta, Kunal Roy
1Division of Medicinal & Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, India.
Abstract:
The present study deals with exploration of lipid peroxidation induction capacity of cyclophosphamide, an anticancer drug, and in vitro evaluation of ascorbic acid as a suppressor of cyclophosphamide induced lipid peroxidation. Goat liver homogenate has been used as the lipid source. This evaluation was done by measuring the malondialdehyde, 4-hydroxy-2-nonenal, reduced glutathione and nitric oxide content of the tissue as markers of lipid peroxidation. The study revealed that ascorbic acid could suppress the drug induced lipid peroxidation to a significant extent.
