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Crystallization and preliminary crystallographic data for the azurin mutant Ala 114 from Pseudomonas aeruginosa
1Department of Inorganic Chemistry, Chalmers University of Technology, Göteborg, Sweden.
Acta Crystallographica. Section B, Structural Science
|February 1, 1992
Abstract:
The site-specific mutant alanine 114 of the blue copper protein azurin from Pseudomonas aeruginosa in Escherichia coli has been crystallized from PEG 4000 in a new crystal form compared to the wild type utilizing the hanging-drop procedure. The crystals are blue well-formed prisms. Monoclinic, P2(1), a = 51.03 (5), b = 83.36 (5), c = 66.30 (6) A and beta = 111.0 (1) degrees. 14,875 reflections up to 2.7 A have been collected using a modified Syntex P2(1) automated four-circle diffractometer.