Related Experiment Video
Updated: Aug 15, 2026
Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
Published on: December 16, 2014
[Detection of apoptosis genes differentially expressed in patients with different degrees of benzene poisoning by
Zhi-Wei Zhao1, Yong-Yi Bi, He-Xi Zhang
1Department of Occupational and Environmental health, School of Public Health, Wuhan University, Wuhan 430071, China.
Objective:
To investigate the differential expression of apoptosis genes in patients with different degrees of benzene poisoning by cDNA microarray.
Methods:
Peripheral mononuclear cells were isolated from seven patients with benzene poisoning of different degrees (suspected 1 case, moderate 2, severe 2), and seven age and sex matched normal control subjects. Total RNA was extracted and purified, followed by reverse transcription to cDNAs with concomitant incorporation of fluorescent dCTP (Cy3 or Cy5). Then 177 genes associated with cell apoptosis were hybridized against the cDNAs probes in microarray. Fluorescent signals were scanned to detect apoptosis genes differentially expressed in patients and normal subjects.
Results:
Forty one genes were found to be differentially expressed between benzene-poisoned patients and normal controls; among the 41 genes, three were up-regulated among patients with mild to moderate degrees of benzene poisoning and one up-regulated among all patients. The total amount of differentially expressed genes of apoptosis decreased with the aggravation of benzene poisoning.
Conclusions:
Differential expression of apoptosis genes was found in patients with benzene poisoning, suggesting a role of altered apoptosis in benzene-induced hematotoxicity.
