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A label-free immunosensor array using single-chain antibody fragments.

Natalija Backmann1, Christian Zahnd, Francois Huber

  • 1National Center of Competence in Research for Nanoscience, Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland.

Proceedings of the National Academy of Sciences of the United States of America
|September 30, 2005
PubMed
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This study presents a novel microcantilever immunosensor using antibody fragments for sensitive antigen detection. The device achieves performance comparable to surface plasmon resonance, enabling detection down to 1 nM concentrations.

Area of Science:

  • Biotechnology and Biosensing
  • Nanotechnology
  • Immunosensors

Background:

  • Microcantilever-based biosensors offer label-free detection capabilities.
  • Surface plasmon resonance (SPR) is a benchmark for high-sensitivity biosensing.
  • Immobilization of bioreceptors in a directed orientation is crucial for optimal sensor performance.

Purpose of the Study:

  • To develop a microcantilever immunosensor utilizing single-chain variable fragment (scFv) antibody fragments.
  • To achieve high sensitivity and performance comparable to SPR using a static deflection mode.
  • To demonstrate the utility of oriented scFv fragments for enhanced antigen detection.

Main Methods:

  • Engineered scFv antibody fragments with a C-terminal cysteine residue for covalent attachment.

Related Experiment Videos

  • Fabrication of a microcantilever array with gold-coated sensor interfaces.
  • Static deflection measurements for label-free, real-time antigen detection.

    • Main Results:

    • The microcantilever immunosensor demonstrated performance comparable to SPR.
    • Specific antigen binding was detected via differential deflection signals.
    • Sensitivity was enhanced to approximately 1 nM using small, oriented scFv fragments.

    Conclusions:

    • Microcantilever immunosensors employing oriented scFv fragments are highly sensitive and effective.
    • This approach offers a viable alternative to SPR for various biosensing applications.
    • The developed sensor system enables precise quantification of antigen concentrations.