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Updated: Aug 15, 2026
Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
Published on: August 31, 2022
[Genotype of isolated strain of hantavirus in Liaoning area]
Xue Dong1, Yong-zhen Zhang, Xin Li
1Microbiologcal Test Section, Shenyang Center for Disease Control and Prevention, Shenyang, China.
Objective:
To isolate hantavirus and to genotype isolated strains of hantavirus (SY 13) in Liaoning province.
Methods:
Hantavirus was isolated by means of cell culture. The G1, G2 fragments of M segments and S segment of the strain of SY 13 were amplified respectively by means of RT-PCR in order to compare and analyze the homology between the sequences of the three isolated gene fragments and the sequences downloaded from GenBank with neighbor-joining through sequence analysis software of DNA Star, Clustal, Phylip, etc.
Results:
SY 13 and HTN showed higher homology, reached up to 79.3%-97.0%; SY 13 and BAO 10, BAO 14, JIANG 13, BAO 9, Q 33 were on the same branch and their homology reached up to 95.0%-97.0%.
Conclusion:
The isolated hantavirus strain in Liaoning area was the same as the one in Heilongjiang area which belongs to HTN H4.
