Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IgE binding to pepsin-digested food extracts.

Jaap H Akkerdaas1, Marjolein Wensing, Riccardo Asero

  • 1Sanquin Research, Department of Immunopathology and Laboratory for Experimental and Clinical Immunology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.

International Archives of Allergy and Immunology
|October 6, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Knowledge of chronic spontaneous urticaria management among Italian general practitioners: Baseline findings from the BRIDGE study.

The World Allergy Organization journal·2026
Same author

<i>In silico</i> prediction and IgE serum screening for potential allergenicity of novel foods-a case of fungal biomass Fermotein®.

Frontiers in allergy·2026
Same author

Angioedema in Primary Care: Disease Course and Its Relation to ACE Inhibitor Use.

International journal of dermatology·2026
Same author

Ranking primary food allergens by specific IgE-binding and clinical relevance in Dutch adults to identify reference protein sets for allergenicity assessment.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2026
Same author

Performance of omalizumab in patients with mast cell-mediated angioedema with and without wheals.

The journal of allergy and clinical immunology. In practice·2026
Same author

D-Dimer Elevation in Spontaneous Urticaria and Bullous Pemphigoid, Two Chronic Inflammatory Skin Diseases.

American journal of hematology·2026
Same journal

Differences in Reported Anaphylaxis Associated With Common Nonsteroidal Anti-inflammatory Drugs: A Pharmacovigilance Study.

International archives of allergy and immunology·2026
Same journal

Association of Serum Galectin-3 Binding Protein with Acute Exacerbation, Inflammation, and Airway Remodeling in Pediatric Asthma.

International archives of allergy and immunology·2026
Same journal

Effectiveness of a Provincial Law to Improve Epinephrine Use for Anaphylaxis in Schools in Alberta, Canada: A Pre-Post Study.

International archives of allergy and immunology·2026
Same journal

Rab27 a regulates dendritic cell immune tolerogenic capacity.

International archives of allergy and immunology·2026
Same journal

Burden of Hereditary Angioedema in Adults: Impact of Disease and Patient Characteristics.

International archives of allergy and immunology·2026
Same journal

Predictors of Treatment Response to Omalizumab in Children with Chronic Spontaneous Urticaria: A Real World Study.

International archives of allergy and immunology·2026
See all related articles

Pepsin-digested food extracts can identify patients with IgE antibodies to stable, potentially severe allergens. This method improves the clinical value of food allergy serology, especially when specific allergens are unknown.

Area of Science:

  • Food allergy diagnostics
  • Immunology
  • Allergen stability

Background:

  • Allergen pepsin resistance, such as for lipid transfer protein and 2S albumin, is linked to severe food allergy symptoms.
  • Component-resolved diagnosis using purified allergens is proposed for risk assessment but purified allergens are often unavailable.
  • This study addresses the need for diagnostic tools when purified allergens are lacking.

Purpose of the Study:

  • To evaluate pepsin-digested whole-food extracts for distinguishing IgE responses to stable versus labile allergens.
  • To assess the utility of this method in identifying sensitization to potentially severe allergens.
  • To enhance the clinical prognostic value of food allergy serology.

Main Methods:

  • Sera from 143 patients with hazelnut/apple allergy symptoms were analyzed.

Related Experiment Videos

  • Radioallergosorbent test and immunoblot analysis were performed using control and pepsin-digested hazelnut/apple extracts.
  • IgE binding to pepsin-resistant and pepsin-susceptible allergens was assessed.

    • Main Results:

    • Pepsin digestion effectively differentiated sensitization to pepsin-resistant from pepsin-susceptible allergens.
    • IgE reactivity to digested extracts correlated with sensitization to stable lipid transfer protein allergens.
    • A small number of false-positive results (2/143) were observed due to soluble pepsin.

    Conclusions:

    • Pepsin-digested food extracts are a practical tool for identifying IgE antibodies against dangerous stable allergens.
    • This approach is particularly valuable for foods lacking identified stable allergens.
    • The method enhances the clinical prognostic value of food allergy serology.