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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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Detection and identification of protein interactions of S100 proteins by ProteinChip technology

Roland Lehmann1, Christian Melle, Niko Escher

  • 1Core Unit Chip Application (CUCA), Institute of Human Genetics and Anthropology, Friedrich-Schiller-University, 07740 Jena, Germany.

Journal of Proteome Research
|October 11, 2005
PubMed

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...