A multilayer membrane amperometric glucose sensor fabricated using planar techniques for large-scale production.

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Biosensors

Background:

• Amperometric glucose sensors are crucial for diabetes management.
• Existing sensors often face challenges in reproducibility and large-scale manufacturing.

Purpose of the Study:

• To develop and characterize a novel multilayer membrane amperometric glucose sensor.
• To assess the sensor's reproducibility, measurement range, stability, and performance in human urine samples.

Main Methods:

• Fabrication of a multilayer membrane sensor using planar techniques.
• Incorporation of a five-layer membrane: gamma-aminopropyltriethoxysilane (gamma-APTES), Nafion, glucose oxidase (GOX), gamma-APTES, and perfluorocarbon polymer (PFCP).
• Electrode configuration includes platinum working electrode (WE), platinum counter electrode (CE), and Ag/AgCl reference electrode (RE).

Main Results:

• The sensor exhibits good reproducibility with a relative standard deviation (R.S.D.) of 42.9% across 82 electrode sets.
• Achieved a wide measurement range of 1.11-111 mM for glucose.
• Demonstrated measurement stability over a 27-day period.
• Showcased very low dispersion (R.S.D.=2.49%, n=10) when measuring glucose in human urine.

Conclusions:

• The developed multilayer membrane amperometric glucose sensor is reproducible and suitable for large-scale production.
• The sensor offers a wide measurement range, good stability, and accurate performance in complex biological samples like urine.
• This technology holds promise for improved glucose monitoring applications.