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Updated: Aug 2, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
High-field spin-label EPR of lipid membranes
Derek Marsh1, Dieter Kurad, Vsevolod A Livshits
1Max-Planck Institut für biophysikalische Chemie, 37070 Göttingen, Germany. dmarsh@gwdg.de
Abstract:
High-field EPR of spin-labelled lipid chains has proved to be an extremely productive means for biophysical investigations of phospholipid bilayer membranes. Results on the following three topics are reviewed: 1. Non-axial ordering of lipid chains in cholesterol-containing membranes; 2. Transmembrane polarity profiles and water penetration in lipid membranes; 3. Role of HF-EPR in multi-frequency spectral simulations to investigate lipid-chain dynamics in membranes. These results are obtained from phosphatidylcholine spin probes with the nitroxide systematically stepped down the sn-2 chain, in fully hydrated bilayer membranes of dimyristoyl phosphatidylcholine (DMPC)-containing cholesterol.
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