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Corn silk induced cyclooxygenase-2 in murine macrophages
Kyung A Kim1, Hyun-Hee Shin, Sang Kyu Choi
1Department of Biological Sciences and the Immunomodulation Research Center, University of Ulsan, Korea.
Abstract:
Stimulation of murine macrophages with corn silk induced cyclooxygenase (COX)-2 with secretion of PGE2. Expression of COX-2 was inhibited by pyrolidine dithiocarbamate (PDTC), and increased DNA binding by nuclear factor kappa B (NF-kappaB), indicating that COX-2 induction proceeds also via the NF-kappaB signaling pathway. A specific inhibitor of COX-2 decreased the expression level of inducible nitric oxide synthase (iNOS) stimulated by corn silk. PGE2 elevated the expression level of iNOS, probably via EP2 and EP4 receptors on the surface of the macrophages.