Related Experiment Video
Updated: Aug 15, 2026
An Efficient Method for Adenovirus Production
Published on: June 10, 2021
[Construction of recombinant adenovirus expressing sCD40L-Ig]
Zhao-lun Li1, Pu-xun Tian, Wu-jun Xue
1Renal Disease Center of First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China. Lizhaolun1@sina.com.cn
Aim:
To construct adenovirus expressing secretory human CD40L-Ig (sCD40 ligand Ig fusion protein).
Methods:
The genes encoding the extracellular domain of human CD40L and IgG Fc were amplified with PCR and inserted into shuttle vector pAdTrack-CMV which then was transformed into E. coli pAdEasy-1-BJ5183 to produce recombinant Ad plasmid. The recombinant plasmid was digested with Pac I and transfected into 293 cells to generate recombinant adenovirus. The recombinant adenovirus was then used in mixed lymphocyte reaction (MLR) to test its function.
Results:
The recombinant adenovirus sCD40L-Ig was produced. It could inhibit the lymphocyte proliferation in MLR.
Conclusion:
The sCD40L-Ig adenovirus is prepared successfully and its inhibition of MLR is confirmed.
