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Published on: September 16, 2018
[Effects of Angelica sinensis polysaccharide on cell-mediated immunity]
Tie-hong Yang1, Min Jia, Qi-bing Mei
1Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China. yangtiehong@sina.com
Aim:
To investigate the effects of Angelica sinensis polysaccharide (AP) on cell-mediated immunity.
Methods:
The lymphocyte proliferation was tested by MTT colormetry; T lymphocyte fraction were separated with immune magnetic beads and the change of CD4(+) T lymphocyte percentage was detected by flow cytometry.
Results:
In the range of 30-300 mg/L, the total AP and its three fractions, ie AP-0, AP-1, AP-2, AP-3, directly stimulated the proliferation of murine splenocytes, and AP-3 significantly enhanced the proliferation of lymphocytes in MLR and T lymphocytes. The percentage of CD4(+) T lymphocytes in total splenocytes was also enhanced by AP-3.
Conclusion:
AP can promote the cell-mediated immunity with T lymphocytes as one of its target cells.
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