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Updated: Aug 15, 2026
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
Published on: October 30, 2016
[Construction and identification of non-replication recombinant vaccinia virus co-expressing human papillomavirus
Wei Huang1, Hou-wen Tian, Jiao Ren
1Department of Gynecologic Oncology, The Affiliated Oncology Hospital, Guangxi Medical University, Nanning 530021, China. huangwei@csco.org.com
Objective:
To generate a human papillomavirus (HPV16) prophylactic and therapeutic vaccine candidate for cervical cancer.
Methods:
HPV16 major capsid protein L1 gene/minor capsid protein L2 gene and HPV16 early E6/E7 genes were inserted into a vaccinia virus expression vector. A strain of non-recombinant vaccinia virus containing the sequences was obtained through a homologous recombination and identified.
Results:
DNA hybridization confirmed that the HPV16L1/L2/E6/E7 genes were integrated into vaccinia virus DNA. Western Blot result showed that full-length L1/L2/E6/E7 proteins were co-expressed in CEF cells infected with the recombinant virus.
Conclusion:
NTVJE6E7CKL1L2 could be taken as a candidate of prophylactic and therapeutic vaccine for HPV-associated tumors and their precancerous transformations.
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