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Storage of platelets in additive solutions: a new method for storage using sodium chloride solution
H Gulliksson1, S Sallander, I Pedajas
1Stockholm Blood Transfusion Service, Sweden.
Transfusion
|June 1, 1992
Summary
Storing platelets in a single container with platelet additive solution (PAS) compromises quality. Using two containers or saline instead of PAS showed no significant difference in platelet storage quality over six days.
Area of Science:
- Transfusion Medicine
- Hematology
- Biotechnology
Background:
- Platelet additive solution (PAS) is used to extend the storage of platelets.
- Optimizing platelet storage conditions is crucial for maintaining their efficacy.
Purpose of the Study:
- To evaluate the in vitro effect of platelet storage in platelet additive solution (PAS).
- To compare platelet storage in one versus two polyolefin containers.
- To assess the impact of PAS versus saline on platelet storage quality.
Main Methods:
- Platelets from pooled buffy coat units were stored for 6 days.
- Evaluated storage in one and two 1000-mL polyolefin containers with PAS.
- Compared PAS with saline for platelet storage in two containers.
Main Results:
- Storage in a single container led to significantly lower pH, pO2, glucose, ATP, and ATP/AMP+ADP+ATP ratio.
- Storage in a single container resulted in higher mean platelet volume, pCO2, lactate, and adenylate kinase activity.
- No significant difference in storage quality was observed between PAS and saline when using two containers.
Conclusions:
- Storage in a single polyolefin container is unsuitable for maintaining platelet quality.
- Platelet additive solution (PAS) demonstrated no specific benefit over saline for 6-day platelet storage in two containers.