Related Experiment Video

Updated: Oct 1, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

[Temperature dependence of the epr spectra of a spin-labeled myosin and its complex with adp]

N V Medvedeva, E K Ruuge, L A Bliumenfel'd

    Biofizika
    |January 1, 1975
    PubMed

    More Related Videos

    Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis08:09

    Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

    Published on: January 7, 2017

    Tuning the Contractility and Deformation Modes of Active Actin-Based Assemblies In Vitro: From Two-Dimensional Active Networks to Liquid Crystal Drops06:48

    Tuning the Contractility and Deformation Modes of Active Actin-Based Assemblies In Vitro: From Two-Dimensional Active Networks to Liquid Crystal Drops

    Published on: July 11, 2025

    Related Concept Videos

    ¹H NMR: Interpreting Distorted and Overlapping Signals01:02

    ¹H NMR: Interpreting Distorted and Overlapping Signals

    Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
    As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
    Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

    Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

    Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
    Atomic Nuclei: Nuclear Spin State Population Distribution01:14

    Atomic Nuclei: Nuclear Spin State Population Distribution

    Near absolute zero temperatures, in the presence of a magnetic field, the majority of nuclei prefer the lower energy spin-up state to the higher energy spin-down state. As temperatures increase, the energy from thermal collisions distributes the spins more equally between the two states. The Boltzmann distribution equation gives the ratio of the number of spins predicted in the spin −½ (N−) and spin +½ (N+) states.
    Spin–Spin Coupling Constant: Overview01:08

    Spin–Spin Coupling Constant: Overview

    In bromoethane, the three methyl protons are coupled to the two methylene protons that are three bonds away. In accordance with the n+1 rule, the signal from the methyl protons is split into three peaks with 1:2:1 relative intensities. The methylene protons appear as a quartet, with the relative intensities of 1:3:3:1.
    Qualitatively, any spin plus-half nucleus polarizes the spins of its electrons to the minus-half state. Consequently, the paired electron in the hydrogen–carbon bond must have a...
    ¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

    ¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

    The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.