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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Quantitation of Bacillus clausii in biological samples by real-time polymerase chain reaction

Mario Perotti1, Nicasio Mancini, Annalisa Cavallero

  • 1Università Vita-Salute San Raffaele and Laboratorio di Microbiologia e Virologia, Diagnostica e Ricerca San Raffaele, Milan, Italy. mario.perotti@hsr.it

Journal of Microbiological Methods
|December 2, 2005
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...