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Pluronic micelles as a tool for low-molecular compound vector delivery into a cell: effect of Staphylococcus aureus
A V Kabanov1, V I Slepnev, L E Kuznetsova
1Russian Research Center of Molecular Diagnostics and Therapy, Moscow.
Abstract:
Micelles of pluronic P85 (poly(oxyethylene)-poly(oxypropylene) block copolymer) are used as microcontainers for in vitro delivery of fluorescein into Jurkat and MDCK cells. In order to target the fluorescein containing micelles into the cell, Staphylococcus aureus enterotoxin B (SEB) is covalently conjugated with a pluronic molecule and the conjugate is incorporated into the micelle content. The incorporation of SEB capable of receptor-mediated endocytosis results in a drastic enhancement of the efficiency of cell loading with the fluorescent dye. This effect is not observed under the conditions (4 degrees C) when endocytosis is abolished.
Insights
Pluronic P85 micelles deliver fluorescein into cells. Conjugating Staphylococcus aureus enterotoxin B (SEB) to micelles enhances cellular uptake via receptor-mediated endocytosis, significantly improving dye delivery efficiency.
Area of Science:
- Biotechnology
- Cell Biology
- Drug Delivery
Background:
- Pluronic P85 (poly(oxyethylene)-poly(oxypropylene) block copolymer) micelles serve as versatile microcontainers.
- Targeted intracellular delivery of molecules remains a challenge in cell biology and biotechnology.
Purpose of the Study:
- To enhance in vitro delivery of fluorescein into Jurkat and MDCK cells using targeted micelles.
- To investigate the role of receptor-mediated endocytosis in enhancing cellular uptake of micelle-encapsulated cargo.
Main Methods:
- Micelles of pluronic P85 were formulated to encapsulate fluorescein.
- Staphylococcus aureus enterotoxin B (SEB) was covalently conjugated with pluronic and incorporated into the micelles.
- Cellular uptake efficiency was assessed in Jurkat and MDCK cells at 37°C and 4°C.
Main Results:
- Micelles incorporating SEB showed drastically enhanced fluorescein delivery into cells.
- The enhanced uptake was dependent on temperature, indicating a role for endocytosis.
- No significant enhancement was observed at 4°C, where endocytosis is inhibited.
Conclusions:
- SEB-functionalized pluronic micelles significantly improve targeted intracellular delivery of fluorescein.
- Receptor-mediated endocytosis is the mechanism driving the enhanced cellular loading.
- This approach offers a promising strategy for targeted delivery applications in biotechnology and cell biology.