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External quality control program for semen analysis: Spanish experience.

C Alvarez1, J A Castilla, J P Ramírez

  • 1Health Sciences Department, University of Jaén, Jaén, Spain. calvarez@ujaen.es

Journal of Assisted Reproduction and Genetics
|December 7, 2005
PubMed
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External quality control for semen analysis in Spain revealed significant variability in key parameters like sperm concentration and motility. These findings underscore the critical need for enhanced andrology testing quality across laboratories.

Area of Science:

  • Andrology
  • Reproductive Medicine
  • Clinical Laboratory Science

Background:

  • Semen analysis is crucial for male fertility assessment.
  • External quality control programs are essential for standardizing laboratory performance.
  • Variability in semen analysis results can impact clinical decisions.

Purpose of the Study:

  • To evaluate the performance of laboratories conducting semen analysis in Spain.
  • To assess the between-laboratory variability for key seminal parameters.
  • To identify areas for improvement in andrology testing.

Main Methods:

  • Distribution of quality control materials for semen analysis.
  • Determination of seminal parameters: concentration, total motility, progressive motility, rapid progressive motility, morphology, and sperm vitality.

Related Experiment Videos

  • Assessment of between-laboratory coefficients of variation (CVs) using various quality control materials.

    • Main Results:

    • Most laboratories used manual semen analysis methods.
    • Reported between-laboratory CVs: concentration (20.8-33.8%), total motility (13.9-19.2%), sperm morphology (54.2-70.2%), and sperm vitality (9.8-41.1%).
    • An inverse relationship was observed between mean sperm percentage and CVs for motility, morphology, and vitality.

    Conclusions:

    • The study highlights significant variability in andrology testing across Spanish laboratories.
    • There is an urgent need to improve the quality and consistency of semen analysis.
    • Standardization and enhanced quality control measures are recommended for andrology laboratories.