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Detection and quantitation of HPV gene expression using real-time PCR

Rashmi Seth1, John Rippin, Li Guo

  • 1Department of Histopathology, Division of Molecular Medicine, Queens Medical Centre, University Hospital, University of Nottingham, UK.

Methods in Molecular Medicine
|December 15, 2005
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...